郁金散对大肠湿热证模型大鼠血清及肠道组织胃肠激素的影响

doi:10.11843/j.issn.0366-6964.2017.06.019

摘要/Abstract

摘要：

探讨郁金散对大肠湿热证模型大鼠血清和肠道组织胃肠激素的影响。Wistar大鼠60只，体重180~220 g，随机分为正常对照组、大肠湿热证模型组、自愈组、郁金散高、中、低剂量治疗组，每组10只。通过自由饮用蜂蜜水；前10 d禁食与饲喂充足饲料并灌胃猪油隔日交替进行；之后5 d每天灌白酒，并置于高温高湿环境（8 h·d^-1）；然后腹腔注射大肠杆菌，24 h后再注射一次建立大肠湿热证大鼠模型。造模成功后，分别给予高（10.4 g·kg^-1）、中（5.2 g·kg^-1）、低（2.6 g·kg^-1）剂量郁金散进行治疗。整个试验过程中观察大鼠的临床症状和体征，记录体重变化，检测血清和回肠、结肠组织中的胃肠激素含量。结果显示：模型大鼠出现便溏、粪便色黄恶臭、肛门红肿、发热等症状和体征。正常组体重逐渐上升，模型大鼠造模期间体重呈现先波状上升、再逐渐下降的趋势，郁金散治疗后体重呈逐渐上升的趋势，其中以高剂量组回升最明显，但各组均无显著性差异（P>0.05）。与正常对照组相比，模型组和自愈组大鼠血清、回肠及结肠组织中胃动素（MTL）、胃泌素（GAS）、P物质（SP）含量极显著或显著升高（P<0.01或P<0.05），血管活性肠肽（VIP）无显著变化，生长抑素（SS）含量极显著降低（P<0.01）；与自愈组相比，各剂量郁金散治疗后，MTL、GAS、SP、VIP和SS都有不同程度的回调，以高剂量组回调最为明显，其中高剂量组大鼠血清、回肠、结肠中MTL、GAS和SP极显著或显著降低（P<0.01或P<0.05），SS极显著或显著升高（P<0.01或P<0.05）。大肠湿热证模型大鼠机体出现了胃肠激素紊乱，表现为MTL、GAS、SP和VIP含量升高，而SS含量降低，郁金散能改善大肠湿热证模型大鼠机体胃肠激素异常水平，且高剂量的治疗效果最好。

Abstract:

This experiment was conducted to explore the effects of Yujin Powder on gastrointestinal hormone in rat model of serum, ileum and colon tissue of large intestine dampness-heat syndrome (LIDHS) rat model. Sixty Wistar rats (180-220 g) were randomly divided into 6 groups with 10 rats in each group: normal control group, model group of LIDHS, self-healing group, high, middle and low dose of Yujin Powder groups. The LIDHS model was established through the following steps: let them drink honey water freely; in the first 10 days, fasting and sufficient feed combined with gavage of lard were alternately performed; the rats were gavaged with wine and put in high temperature and high humidity environment (8 h·d^-1) during the following 5 days,and then they were intraperitoneally injected with Escherichia coli once again after 24 hours. After establishment of the model, the rats were treated with high (10.4 g·kg^-1), middle (5.2 g·kg^-1) and low-dose (2.6 g·kg^-1) Yujin Powder, respectively. During the experiment, the clinical symptoms and signs of rats were observed, weights were recorded and the contents of gastrointestinal hormone in serum and ileum and colon tissue were detected. Results were as follows: Model rats appeared loose stools. Their feces were yellow and rotten. The anus was red and swollen and so on. The weights of normal group were gradually increase. The weights of model rats showed a trend from fluctuating rise to gradually decline. After treatment with all doses of Yujin Powder, the weights gradually rose, especially in the high dose group, but no significant different was found. Compared with normal group, the contents of motilin (MTL), gastrin (GAS), substance P (SP) increased significantly (P<0.01 or P<0.05) in serum, ileum and colon tissue in model and self-healing groups. Somatostatin (SS) decreased significantly (P<0.01) and vasoactive intestinal peptide (VIP) showed no significant changes. Compared with self-healing group, after treatment with various doses of Yujin Powder, the above-mentioned indexes tend to normal condition to varying degrees, especially in the high dose group. In the high dose group, the contents of MTL, GAS and SP decreased significantly (P<0.01 or P<0.05), and SS increased significantly (P<0.01 or P<0.05) in serum and ileum and colon tissue. It was demonstrated that the gastrointestinal hormones presented disorder in LIDHS rat model, that the contents of MTL, GAS, SP and VIP significantly increased and SS declined in LIDHS rat model. Yujin Powder could significantly adjust the disorder state of gastrointestinal hormone in LIDHS model rat and the effects of high dose was the best.

中图分类号:

S853.9

文艳巧, 姚万玲, 杨朝雪, 马琪, 张晓松, 纪鹏, 华永丽, 魏彦明. 郁金散对大肠湿热证模型大鼠血清及肠道组织胃肠激素的影响[J]. 畜牧兽医学报, 2017, 48(6): 1140-1149.

WEN Yan-qiao, YAO Wan-ling, YANG Chao-xue, MA Qi, ZHANG Xiao-song, JI Peng, HUA Yong-li, WEI Yan-ming. Effects of Yujin Powder on Gastrointestinal Hormone in Serum and Intestinal Tissue of Large Intestine Dampness-heat Syndrome Rat Model[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1140-1149.

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